Background: The emergence of antibacterial resistance and ESBL-producing Escherichia coli recovered from urinary tract infections (UTI) are known as important health problems in different regions, therefore therapeutic options for these infections are limited. The aim of this study was the detection of blaTEM, blaSHV and blaCTX-M genesamong Escherichia colistrains isolated from Children Medical Center hospital, Tehran, Iran.Methods: This study was conducted on 100 Escherichia coli isolates from urine specimens of patients with UTI who referred to Children Medical Center, Tehran, Iran from November 2012 and July 2013. Antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method according to CLSI guidelines. The blaCTX-M, blaTEM and blaSHVgenes were detected by PCR and sequencing methods.Results: The resistance rate of isolates to Gentamicin, Imipenem, Nitrofurantoin, Cefotaxime, Ceftazidime, Amikacin, Cefepime, Piperacillin/Tazobactam, Cotrimoxazole, Cefixime andCephalothin, were 25 (25%), 0 (0%), 7 (7%), 51 (51%), 24 (24%), 2 (2%), 6 (6%), 2 (2%), 79 (79%), 36 (36%) and 43 (43%) respectively. In this study, imipenemwas more active than other antibiotics. The existence of blaTEM- 1 and blaCTX-M-15was detected in 69 (69%) and 74 (74%) of isolates respectively, while blaSHVgene was not detected.Conclusion: The prevalence of ESBL-producing E. coli detected in this study is of great concern and highlights the need of infection control measures including antibacterial management and prompt identification of ESBL-producing isolates.